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20 December 2017 Femtosecond laser surgery of two-cell mouse embryos: effect on viability, development, and tetraploidization
Alina A. Osychenko, Alexandr D. Zalessky, Andrey N. Kostrov, Anastasia V. Ryabova, Alexander S. Krivokharchenko, Victor A. Nadtochenko
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Journal of Biomedical Optics, Vol. 22, Issue 12, 125006 (December 2017). https://doi.org/10.1117/1.JBO.22.12.125006
Abstract
The effect of the laser pulse energy and total expose of the energy incident on the embryo blastomere fusion probability was investigated. The probability of the four different events after laser pulse was determined: the fusion of two blastomeres with the following formation of tetraploid embryo, the destruction of the first blastomere occurs, the second blastomere conservation remains intact, the destruction and the death of both cells; two blastomeres were not fused, and no morphological changes occurred. We report on viability and quality of the embryo after laser surgery as a function of the laser energy incident. To characterize embryo quality, the probability of the blastocyst stage achievement was estimated and the blastocyst cells number was calculated. Blastocoel formation is the only event of morphogenesis in the preimplantation development of mammals, so we assumed it as an indicator of the time of embryonic “clocks” and observed it among fused and control embryos. The blastocoel formation time is the same for fused and control embryos. It indicates that embryo clocks were not affected due to blastomere fusion. Thus, the analysis of the fluorescence microscopic images of nuclei in the fused embryo revealed that nuclei fusion does not occur after blastomere fusion.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2017/$25.00 © 2017 SPIE
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Alina A. Osychenko, Alexandr D. Zalessky, Andrey N. Kostrov, Anastasia V. Ryabova, Alexander S. Krivokharchenko, and Victor A. Nadtochenko "Femtosecond laser surgery of two-cell mouse embryos: effect on viability, development, and tetraploidization," Journal of Biomedical Optics 22(12), 125006 (20 December 2017). https://doi.org/10.1117/1.JBO.22.12.125006
Received: 10 May 2017; Accepted: 21 November 2017; Published: 20 December 2017
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KEYWORDS
Femtosecond phenomena
Laser therapeutics
Fusion energy
Surgery
Pulsed laser operation
Absorption
Luminescence
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