Negative dielectrophoresis spectroscopy for rare analyte quantification in biological samples

Syed A. M. Kirmani; Fleming Dackson Gudagunti; Logeeshan Velmanickam; Dharmakeerthi Nawarathna; Ivan T. Lima

doi:10.1117/1.JBO.22.3.037006

30 March 2017 Negative dielectrophoresis spectroscopy for rare analyte quantification in biological samples

Syed A. M. Kirmani, Fleming Dackson Gudagunti, Logeeshan Velmanickam, Dharmakeerthi Nawarathna, Ivan T. Lima

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Journal of Biomedical Optics, Vol. 22, Issue 3, 037006 (March 2017). https://doi.org/10.1117/1.JBO.22.3.037006

Abstract

We propose the use of negative dielectrophoresis (DEP) spectroscopy as a technique to improve the detection limit of rare analytes in biological samples. We observe a significant dependence of the negative DEP force on functionalized polystyrene beads at the edges of interdigitated electrodes with respect to the frequency of the electric field. We measured this velocity of repulsion for 0% and 0.8% conjugation of avidin with biotin functionalized polystyrene beads with our automated software through real-time image processing that monitors the Rayleigh scattering from the beads. A significant difference in the velocity of the beads was observed in the presence of as little as 80 molecules of avidin per biotin functionalized bead. This technology can be applied in the detection and quantification of rare analytes that can be useful in the diagnosis and the treatment of diseases, such as cancer and myocardial infarction, with the use of polystyrene beads functionalized with antibodies for the target biomarkers.

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Syed A. M. Kirmani, Fleming Dackson Gudagunti, Logeeshan Velmanickam, Dharmakeerthi Nawarathna, and Ivan T. Lima "Negative dielectrophoresis spectroscopy for rare analyte quantification in biological samples," Journal of Biomedical Optics 22(3), 037006 (30 March 2017). https://doi.org/10.1117/1.JBO.22.3.037006

Received: 29 November 2016; Accepted: 14 March 2017; Published: 30 March 2017

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