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21 June 2018 Dual-dimensional microscopy: real-time in vivo three-dimensional observation method using high-resolution light-field microscopy and light-field display
Jonghyun Kim, Seokil Moon, Youngmo Jeong, Changwon Jang, Youngmin Kim, Byoungho Lee
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Journal of Biomedical Optics, Vol. 23, Issue 6, 066502 (June 2018). https://doi.org/10.1117/1.JBO.23.6.066502
Abstract
Here, we present dual-dimensional microscopy that captures both two-dimensional (2-D) and light-field images of an in-vivo sample simultaneously, synthesizes an upsampled light-field image in real time, and visualizes it with a computational light-field display system in real time. Compared with conventional light-field microscopy, the additional 2-D image greatly enhances the lateral resolution at the native object plane up to the diffraction limit and compensates for the image degradation at the native object plane. The whole process from capturing to displaying is done in real time with the parallel computation algorithm, which enables the observation of the sample’s three-dimensional (3-D) movement and direct interaction with the in-vivo sample. We demonstrate a real-time 3-D interactive experiment with Caenorhabditis elegans.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Jonghyun Kim, Seokil Moon, Youngmo Jeong, Changwon Jang, Youngmin Kim, and Byoungho Lee "Dual-dimensional microscopy: real-time in vivo three-dimensional observation method using high-resolution light-field microscopy and light-field display," Journal of Biomedical Optics 23(6), 066502 (21 June 2018). https://doi.org/10.1117/1.JBO.23.6.066502
Received: 14 February 2018; Accepted: 30 May 2018; Published: 21 June 2018
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Cited by 2 scholarly publications.
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KEYWORDS
3D displays
Microscopy
3D image processing
In vivo imaging
Fourier transforms
Image resolution
Video
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