Optical model of the murine lung to optimize pulmonary illumination

Madeleine S. Durkee; Fatemeh Nooshabadi; Jeffrey D. Cirillo; Kristen C. Maitland

doi:10.1117/1.JBO.23.7.071208

23 March 2018 Optical model of the murine lung to optimize pulmonary illumination

Madeleine S. Durkee, Fatemeh Nooshabadi, Jeffrey D. Cirillo, Kristen C. Maitland

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Journal of Biomedical Optics, Vol. 23, Issue 7, 071208 (March 2018). https://doi.org/10.1117/1.JBO.23.7.071208

Abstract

We describe a Monte Carlo model of the mouse torso to optimize illumination of the mouse lung for fluorescence detection of low levels of pulmonary pathogens, specifically Mycobacterium tuberculosis. After validation of the simulation with an internally illuminated optical phantom, the entire mouse torso was simulated to compare external and internal illumination techniques. Measured optical properties of deflated mouse lungs were scaled to mimic the diffusive properties of inflated lungs in vivo. Using the full-torso model, a 2 × to 3 × improvement in average fluence rate in the lung was seen for dorsal compared with ventral positioning of the mouse with external illumination. The enhancement in average fluence rate in the lung using internal excitation was 40 × to 60 × over external illumination in the dorsal position. Parameters of the internal fiber optic source were manipulated in the model to guide optimization of the physical system and experimental protocol for internal illumination and whole-body detection of fluorescent mycobacteria in a mouse model of infection.

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Madeleine S. Durkee, Fatemeh Nooshabadi, Jeffrey D. Cirillo, and Kristen C. Maitland "Optical model of the murine lung to optimize pulmonary illumination," Journal of Biomedical Optics 23(7), 071208 (23 March 2018). https://doi.org/10.1117/1.JBO.23.7.071208

Received: 2 October 2017; Accepted: 1 March 2018; Published: 23 March 2018

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