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25 April 2019 Methamphetamine-induced apoptosis in glial cells examined under marker-free imaging modalities
Lianna Y. D’Brant, Habben Desta, Ting Chean Khoo, Anna V. Sharikova, Supriya D. Mahajan, Alexander Khmaladze
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Funded by: National Institute on Drug Abuse (NIDA)
Journal of Biomedical Optics, Vol. 24, Issue 4, 046503 (April 2019). https://doi.org/10.1117/1.JBO.24.4.046503
Abstract
We used phase microscopy and Raman spectroscopic measurements to assess the response of in vitro rat C6 glial cells following methamphetamine treatment in real time. Digital holographic microscopy (DHM) and three-dimensional (3-D) tomographic nanoscopy allow measurements of live cell cultures, which yield information about cell volume changes. Tomographic phase imaging provides 3-D information about the refractive index distribution associated with the morphology of biological samples. DHM provides similar information, but for a larger population of cells. Morphological changes in cells are associated with alterations in cell cycle and initiation of cell death mechanisms. Raman spectroscopy measurements provide information about chemical changes within the cells. Our Raman data indicate that the chemical changes in proteins preceded morphological changes, which were seen with DHM. Our study also emphasizes that tomographic phase imaging, DHM, and Raman spectroscopy are imaging tools that can be utilized for noninvasive simultaneous monitoring of morphological and chemical changes in cells during apoptosis and can also be used to monitor other dynamic cell processes.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Lianna Y. D’Brant, Habben Desta, Ting Chean Khoo, Anna V. Sharikova, Supriya D. Mahajan, and Alexander Khmaladze "Methamphetamine-induced apoptosis in glial cells examined under marker-free imaging modalities," Journal of Biomedical Optics 24(4), 046503 (25 April 2019). https://doi.org/10.1117/1.JBO.24.4.046503
Received: 5 January 2019; Accepted: 5 April 2019; Published: 25 April 2019
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Cited by 16 scholarly publications.
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KEYWORDS
Cell death
Raman spectroscopy
Glia
3D image processing
Digital holography
Proteins
Control systems
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