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1 July 2003 Mechanisms of membrane potential sensing with second-harmonic generation microscopy
Thomas Pons, Laurent Moreaux, Olivier Mongin, Mireille H. Blanchard-Desce, Jerome Mertz
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Journal of Biomedical Optics, Vol. 8, Issue 3, (July 2003). https://doi.org/10.1117/1.1581871
We characterize the transmembrane voltage response of a novel second-harmonic generation (SHG) marker using a screening protocol with giant unilamellar vesicles. Two mechanisms are found to contribute to the voltage response: (1) an electro-optic-induced alteration of the molecular hyperpolarizability and (2) an electric-field-induced alteration of the degree of molecular alignment. We quantify the relative weights and of these contributions and provide an upper limit to their response time, which is found to be submillisecond. The identification of two voltage response mechanisms leads to new strategies for the molecular design of membrane potential markers.
©(2003) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Thomas Pons, Laurent Moreaux, Olivier Mongin, Mireille H. Blanchard-Desce, and Jerome Mertz "Mechanisms of membrane potential sensing with second-harmonic generation microscopy," Journal of Biomedical Optics 8(3), (1 July 2003). https://doi.org/10.1117/1.1581871
Published: 1 July 2003
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KEYWORDS
Second-harmonic generation
Electro optics
Microscopy
Line scan image sensors
Molecules
Switching
Polarization
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