Open Access
14 August 2018 Advances in nonlinear optical microscopy for biophotonics
Rui Li, Xinxin Wang, Yi Zhou, Huan Zong, Maodu Chen, Mengtao Sun
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Abstract
This article reviews recent advances in nonlinear optical microscopy for biophotonics, including stimulated Raman scattering (SRS), coherent anti-Stokes Raman scattering (CARS), two-photon excited fluorescence (TPEF), second harmonic generation (SHG), and sum frequency generation (SFG). We first introduce the principles and applications of SRS, CARS, TPEF, SHG, and SFG and their individual applications for biophotonics. We then introduce the combination of SRS, CARS, TPEF, SHG, and SFG microscopy for biophotonics. Our review not only summarizes the recent advances in nonlinear optical microscopy but also can deepen the understanding of the combination of these types of nonlinear optical microscopy for biophotonics.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE) 1934-2608/2018/$25.00 © 2018 SPIE
Rui Li, Xinxin Wang, Yi Zhou, Huan Zong, Maodu Chen, and Mengtao Sun "Advances in nonlinear optical microscopy for biophotonics," Journal of Nanophotonics 12(3), 033007 (14 August 2018). https://doi.org/10.1117/1.JNP.12.033007
Received: 6 June 2018; Accepted: 30 July 2018; Published: 14 August 2018
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Cited by 27 scholarly publications.
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KEYWORDS
Microscopy

Second-harmonic generation

Optical microscopy

Biomedical optics

Tissues

Microscopes

Luminescence

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