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8 March 2024 Low-cost reversible tandem lens mesoscope for brain imaging in rodents
Ashly Jose, Pang Ying Cheung, Zahra Laouby, Frédérique Vanholsbeeck, Juliette E. Cheyne
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Neurophotonics, Vol. 11, Issue 1, 014306 (March 2024). https://doi.org/10.1117/1.NPh.11.1.014306
Abstract

Significance

The development of imaging systems that are cost-efficient and modular is essential for modern neuroscience research.

Aim

In the current study, we designed, developed, and characterized a low-cost reversible tandem lens mesoscope for brain imaging in rodents.

Approach

Using readily available components, we assembled a robust imaging system that is highly efficient and cost-effective. We developed a mesoscope that offers high-resolution structural and functional imaging with cost-effective lenses and CMOS camera.

Results

The reversible tandem lens configuration of the mesoscope offers two fields of view (FOVs), which can be achieved by swapping the objective and imaging lenses. The large FOV configuration of 12.6×10.5 mm provides a spatial resolution up to 4.92 μm, and the small FOV configuration of 6×5 mm provides a resolution of up to 2.46 μm. We demonstrate the efficiency of our system for imaging neuronal calcium activity in both rat and mouse brains in vivo.

Conclusions

The careful selection of the mesoscope components ensured its compactness, portability, and versatility, meaning that different types of samples and sample holders can be easily accommodated, enabling a range of different experiments both in vivo and in vitro. The custom-built reversible FOV mesoscope is cost-effective and was developed for under US$10,000 with excellent performance.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Citation Download Citation
Ashly Jose, Pang Ying Cheung, Zahra Laouby, Frédérique Vanholsbeeck, and Juliette E. Cheyne "Low-cost reversible tandem lens mesoscope for brain imaging in rodents," Neurophotonics 11(1), 014306 (8 March 2024). https://doi.org/10.1117/1.NPh.11.1.014306
Received: 25 April 2023; Accepted: 9 February 2024; Published: 8 March 2024
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KEYWORDS
Brain
Neuroimaging
Imaging systems
Spatial resolution
Cameras
Lenses
Calcium
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