Fluorescence micro-optical sectioning tomography using acousto-optical deflector-based confocal scheme

Xiaoli Qi; Tao Yang; Longhui Li; Jiancun Wang; Shaoqun Zeng; Xiaohua Lv

doi:10.1117/1.NPh.2.4.041406

15 October 2015 Fluorescence micro-optical sectioning tomography using acousto-optical deflector-based confocal scheme

Xiaoli Qi, Tao Yang, Longhui Li, Jiancun Wang, Shaoqun Zeng, Xiaohua Lv

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Neurophotonics, Vol. 2, Issue 4, 041406 (October 2015). https://doi.org/10.1117/1.NPh.2.4.041406

Abstract

Fluorescent labeling has opened up the possibility of clarifying the complex distribution and circuit wiring of specific neural circuits for particular functions. To acquire the brain-wide fluorescently labeled neural wiring, we have previously developed the fluorescence micro-optical sectioning tomography imaging system. This employs simultaneous mechanical sectioning and confocal imaging of the slices, and is capable of acquiring the image dataset of a centimeter-sized whole-mouse brain at a voxel resolution of 1 μm. We analyze the key optical considerations for the use of an acousto-optical deflector (AOD) scanner-based confocal detection scheme in this system. As a result, the influence of confocal detection, the imaging site during sectioning, and AOD fast scan mode on signal-to-background noise ratio are described. It is shown that mechanical sectioning to separate the slice and optical sectioning by confocal detection should be combined to maximize background suppression in simultaneous fast scan imaging while sectioning system setup.

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Xiaoli Qi, Tao Yang, Longhui Li, Jiancun Wang, Shaoqun Zeng, and Xiaohua Lv "Fluorescence micro-optical sectioning tomography using acousto-optical deflector-based confocal scheme," Neurophotonics 2(4), 041406 (15 October 2015). https://doi.org/10.1117/1.NPh.2.4.041406

Published: 15 October 2015

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