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We present the visualization of the mouse cerebellum and adjacent brainstem using a serial optical coherence scanner, which integrates a vibratome slicer and polarization-sensitive optical coherence tomography for ex vivo imaging. The scanner provides intrinsic optical contrasts to distinguish the cerebellar cortical layers and white matter. Images from serial scans reveal the large-scale anatomy in detail and map the nerve fiber pathways in the cerebellum and brainstem. By incorporating a water-immersion microscope objective, we also present high-resolution tiled images that delineate fine structures in the cerebellum and brainstem.
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Chao J. Liu, Kristen E. Williams, Harry T. Orr, Taner Akkin, "Visualizing and mapping the cerebellum with serial optical coherence scanner," Neurophoton. 4(1) 011006 (30 September 2016) https://doi.org/10.1117/1.NPh.4.1.011006