We theoretically demonstrate a high-resolution fluorescence imaging scheme with configurations of oblique right angle optical path and ring-shaped excitation and fluorescent beams. The oblique right angle optical path configuration with two orthogonally aligned objective lenses over the specimen. It has strong abilities to eliminate the imaging stacking process in depth direction, to enhance the fluorescent collection efficiency, to improve the resolution, and to simplify the architecture of the fast fluorescent imaging system. Based on the Fourier domain model simulation, it is verified that the ring-shaped beams can further improve the imaging depth and resolution. The axial resolution can be optimized to 472 nm, the lateral resolution can be improved to 275 nm; meanwhile, the system can have an imaging depth of 398 μm. |
ACCESS THE FULL ARTICLE
No SPIE Account? Create one
Luminescence
Imaging systems
Point spread functions
Objectives
Gaussian beams
Image resolution
Spatial frequencies