Dr. Elsa M. Materón Profile

Dr. Elsa M. Materón

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 31 May 2023 Poster + Paper

Functionalization of nanostructured surfaces for optical sensor platforms

Vicente Silva Mattos, Jarbas Caiado de Castro Neto, Francisco Eduardo Gontijo Guimarães, Raphael Antonio Caface, Elsa M. Materón

Proceedings Volume 12572, 1257212 (2023) https://doi.org/10.1117/12.2664866

KEYWORDS: Antibodies, Nanostructures, Laser nanostructuring, Fluorophores, Molecules, Fluorescence, Nanostructuring, Metals, Nanolithography, Optical sensors

Read Abstract +

In every field of science, new techniques for target molecule detection are increasingly required. This work presents itself as a method for developing new sensors that can detect target molecules even in samples with low concentrations. The aim of the current research project is to create an optical sensor substrate that is precise and specific for fluorescent or fluorescently marked targets. We produced nanostructured surfaces on bulk silver substrates using a femtosecond pulsed laser (Laser Libra Ti:Saphira from Coherent). These surfaces have a larger superficial area, which increases the fluorescence of molecules nearby by due to the surface plasmon resonance, in the effect of metal-enhanced fluorescence (MEF). The COVID virus spike antibody (SARS-CoV-2(2019-nCoV)) and a secondary fluorescently marked antibody (Alexa Fluor™ 633 goat anti-human IgG (H+L)) was used to functionalize the nanostructures. Initial results indicated that the functionalization process was successful in achieving our initial goals, presenting the proposal's methods as an effective route for the development of biosensors. The primary antibody was initially detected in a very low concentration (0.0525 ng/uL), and the fluorescent signal was enhanced on the nanostructured portion of the surface by 6.3 times more than it was on the surface without modification.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years