This manuscript analyzes the optical properties of dystrophic mice legs, which have been obtained by Spatial Frequency Domain Imaging (SFDI). We used a custom-built SFDI system with spectrometric capabilities so that wavelength-resolved absorption (μa) and scattering (μ′s) coefficients can be calculated. Samples were measured sequentially at ten different frequencies to find their frequency-dependent diffuse reflectance. Additionally, the Monte Carlo method has been applied to generate a Look-Up Table (LUT) to speed up the estimation of the optical parameters, with the GPU-accelerated version of Monte Carlo for Multi-Layered tissues (MCML), CUDAMCML. We found that the diffuse reflectance (Rd) has a different behavior in terms of the wavelength (λ), which gave rise to different values of µa and μ′s in terms of λ. Given that the μa is related to the chemical composition, the differences in wavelength could be used to quantify the presence of chemical components in the samples and, the μ′s, which relates to the internal structure, can be utilized to identify dystrophy centers inside the mice leg.
In this work, intensity and polarization OCT were applied, simultaneously, to alpha-sarcoglycan deficit mice models, and a new visualization technique was implemented, based on encoding the attenuation and birefringence values in the HSV color space. Our samples consisted of 14 ex-vivo mice quadriceps at different disease stages (one, three, and six-month-old mice) and four healthy ones for reference. The healthy muscles present a different birefringence distribution to the dystrophic ones, while attenuation values for both kinds of samples lay in the same range. Nevertheless, the attenuation provides an increase in contrast and textural features that are not visible by only using birefringence, while the latter, encoded in the H coordinate, helps to easily identify damage inside the samples by color.
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