Single-shot full-field reflection-mode off-axis quantitative phase microscopy with temporal focusing

Yuechuan Lin; Peter So; Zahid Yaqoob

doi:10.1117/12.3000099

13 March 2024 Single-shot full-field reflection-mode off-axis quantitative phase microscopy with temporal focusing

Yuechuan Lin, Peter So, Zahid Yaqoob

Proceedings Volume PC12852, Quantitative Phase Imaging X; PC128520D (2024) https://doi.org/10.1117/12.3000099
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We demonstrate a single-shot full-field reflection-mode off-axis quantitative phase microscopy with temporal focusing. The new imaging system is capable of achieving diffraction-limited optical sectioning quantitative phase imaging without any spatial or angular multiplexing. It has high potential to achieve ultrafast imaging with camera-limited frame rate. We show that the QPM can measure the depth-dependent intracellular scattering dynamics of cells and reveals 3D structure of tissues with different phenotypes at sub-cellular resolution.

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Yuechuan Lin, Peter So, and Zahid Yaqoob "Single-shot full-field reflection-mode off-axis quantitative phase microscopy with temporal focusing", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC128520D (13 March 2024); https://doi.org/10.1117/12.3000099

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KEYWORDS

Microscopy

Reflection

Biological imaging

Light sources and illumination

Multiplexing

Phase imaging

Tissue optics

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