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13 March 2024 Ultrasensitive detection of tracer contaminants for point-of-care tests
Eilon Sherman
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Proceedings Volume PC12861, Frontiers in Biological Detection: From Nanosensors to Systems XVI; PC1286107 (2024) https://doi.org/10.1117/12.3002563
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Several scenarios require very fast (<5min) detection of various pathogens at the point of care or need (POC/N), including screening for highly infectious pathogens, life threatening conditions, etc. Gold standard and accurate techniques, including culturing and qPCR, remain too slow for such applications, and are relatively expensive and complicated to use at the POC/N. We developed a method for detecting genomic pathogenic molecules at the single molecule level, thus eliminating the need to amplify these molecules. The method relies on fluorescent hybridization probes that bind specific genes of interest. Upon target binding, the probes generate a FRET signal that undergoes time-dependent modulation through reversible frustration of the FRET process. Unbound probes and other background components remain unmodulated, and their signal is filtered out in the frequency domain, allowing sensitive signal detection from single probe molecules bound to the target genes. The method is fast (~2min) and accurate (>95% sensitivity and specificity), as demonstrated for STD causing pathogens. The method is also general and can be expanded to additional pathogens and media of interest.
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Eilon Sherman "Ultrasensitive detection of tracer contaminants for point-of-care tests", Proc. SPIE PC12861, Frontiers in Biological Detection: From Nanosensors to Systems XVI, PC1286107 (13 March 2024); https://doi.org/10.1117/12.3002563
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KEYWORDS
Molecules
Pathogens
Point-of-care devices
Diagnostics
Signal detection
Fluorescence resonance energy transfer
Genomics
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