HeLa cells response to photodynamic treatment with Radachlorin at various irradiation parameters

A. V. Belashov; A. A. Zhikhoreva; T. N. Belyaeva; E. S. Kornilova; N. V. Petrov; A. V. Salova; I. V. Semenova; O. S. Vasyutinskii

doi:10.1117/12.2287208

28 July 2017 HeLa cells response to photodynamic treatment with Radachlorin at various irradiation parameters

A. V. Belashov, A. A. Zhikhoreva, T. N. Belyaeva, E. S. Kornilova, N. V. Petrov, A. V. Salova, I. V. Semenova, O. S. Vasyutinskii

Author Affiliations +

Proceedings Volume 10414, Advances in Microscopic Imaging; 104140U (2017) https://doi.org/10.1117/12.2287208
Event: European Conferences on Biomedical Optics, 2017, Munich, Germany

Abstract

Measurements of average phase shifts introduced by living HeLa cells to probe wave front were carried out. Variations of this value were monitored in the course of morphological changes caused by photodynamic treatment at various irradiation doses. Observations of changes in living cells were also performed by means of far field optical microscopy and confocal fluorescent microscopy. Quantitative analysis of the data obtained shows that average phase shift introduced by the cells may either increase or decrease depending upon major parameters of the treatment.

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A. V. Belashov, A. A. Zhikhoreva, T. N. Belyaeva, E. S. Kornilova, N. V. Petrov, A. V. Salova, I. V. Semenova, and O. S. Vasyutinskii "HeLa cells response to photodynamic treatment with Radachlorin at various irradiation parameters", Proc. SPIE 10414, Advances in Microscopic Imaging, 104140U (28 July 2017); https://doi.org/10.1117/12.2287208

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