3D fluorescence microscopy with multi-shot coded aperture measurements (Conference Presentation)

Hsiou-Yuan Liu; Laura Waller

doi:10.1117/12.2292478

14 March 2018 3D fluorescence microscopy with multi-shot coded aperture measurements (Conference Presentation)

Hsiou-Yuan Liu, Laura Waller

Proceedings Volume 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV; 104991B (2018) https://doi.org/10.1117/12.2292478
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Coded aperture imaging for 3D reconstructions have been applied in both photography and microscopy to successfully recover depth information from images captured with a patterned pupil plane. Generally, single-shot coded aperture microscopy methods require the 3D sample to be extremely sparse and with no overlapping features. Here, we extend coded aperture microscopy methods to multi-shot 3D fluorescent imaging with wave-optical forward models, enabling thicker and denser 3D samples to be faithfully reconstructed with efficient data capture via compressed sensing and advanced inverse algorithms.. We demonstrate successful reconstruction of a 500um thick brine shrimp sample. We further study the minimum required number of codes to capture full fluorescent information under wide-field illumination The experiments are carried out with a 4f system and a spatial light modulator in the Fourier (aperture) domain to display the codes.

Conference Presentation

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Hsiou-Yuan Liu and Laura Waller "3D fluorescence microscopy with multi-shot coded aperture measurements (Conference Presentation)", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104991B (14 March 2018); https://doi.org/10.1117/12.2292478

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KEYWORDS

3D image processing

3D metrology

3D modeling

Coded apertures

Microscopy

Coded aperture imaging

Data modeling

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