A molecular-sized optical logic circuit for digital modulation of a fluorescence signal

Takahiro Nishimura; Karin Tsuchida; Yusuke Ogura; Jun Tanida

doi:10.1117/12.2299499

5 March 2018 A molecular-sized optical logic circuit for digital modulation of a fluorescence signal

Takahiro Nishimura, Karin Tsuchida, Yusuke Ogura, Jun Tanida

Proceedings Volume 10714, Third International Conference on Photonics Solutions (ICPS2017); 1071409 (2018) https://doi.org/10.1117/12.2299499
Event: Third International Conference on Photonic Solutions, 2017, Pattaya, Thailand

Abstract

Fluorescence measurement allows simultaneous detection of multiple molecular species by using spectrally distinct fluorescence probes. However, due to the broad spectra of fluorescence emission, the multiplicity of fluorescence measurement is generally limited. To overcome this limitation, we propose a method to digitally modulate fluorescence output signals with a molecular-sized optical logic circuit by using optical control of fluorescence resonance energy transfer (FRET). The circuit receives a set of optical inputs represented with different light wavelengths, and then it switches high and low fluorescence intensity from a reporting molecule according to the result of the logic operation. By using combinational optical inputs in readout of fluorescence signals, the number of biomolecular species that can be identified is increased. To implement the FRET-based circuits, we designed two types of basic elements, YES and NOT switches. An YES switch produces a high-level output intensity when receiving a designated light wavelength input and a low-level intensity without the light irradiation. A NOT switch operates inversely to the YES switch. In experiments, we investigated the operation of the YES and NOT switches that receive a 532-nm light input and modulate the fluorescence intensity of Alexa Fluor 488. The experimental result demonstrates that the switches can modulate fluorescence signals according to the optical input.

Citation Download Citation

Takahiro Nishimura, Karin Tsuchida, Yusuke Ogura, and Jun Tanida "A molecular-sized optical logic circuit for digital modulation of a fluorescence signal", Proc. SPIE 10714, Third International Conference on Photonics Solutions (ICPS2017), 1071409 (5 March 2018); https://doi.org/10.1117/12.2299499

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