Effect of light-assisted drying (LAD) on protein functionality

Madison A. Young; Gloria D. Elliott; Susan R. Trammell

doi:10.1117/12.2508178

26 February 2019 Effect of light-assisted drying (LAD) on protein functionality

Madison A. Young, Gloria D. Elliott, Susan R. Trammell

Proceedings Volume 10869, Optics and Biophotonics in Low-Resource Settings V; 108690U (2019) https://doi.org/10.1117/12.2508178
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Light-Assisted Drying (LAD) is a novel biopreservation technique which allows proteins to be immobilized in a dry, amorphous solid at room temperature. Indicator proteins are used in a variety of diagnostic assays ranging from highthroughput 96-well plates to new microfluidic devices. A challenge in the development of protein-based assays is preserving the structure of the protein during production and storage of the assay, as the structure of the protein is responsible for its functional activity. Freeze-drying or freezing are currently the standard for the preservation of proteins, but these methods are expensive and can be challenging in some environments due to a lack of available infrastructure. An inexpensive, simple processing method that enables supra-zero temperature storage of proteins used in assays is needed. Light-assisted drying offers a relatively inexpensive method for drying samples. Proteins suspended in a trehalose solution are dehydrated using near-infrared laser light. The laser radiation speeds drying and as water is removed the sugar forms a protective matrix. In this set of studies we investigate the effect varying protein concentration and protein size on EMC. We also test the functionality of a model protein, lysozyme, after LAD processing compared to air drying, samples incubated at a temperature comparable to LAD, and a control solution kept at 8°C.

Conference Presentation

Citation Download Citation

Madison A. Young, Gloria D. Elliott, and Susan R. Trammell "Effect of light-assisted drying (LAD) on protein functionality", Proc. SPIE 10869, Optics and Biophotonics in Low-Resource Settings V, 108690U (26 February 2019); https://doi.org/10.1117/12.2508178

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available