Improving qualitative phase imaging using error eliminating assist of two-steps phase shifting method in digital holography system

Chantira Boonsri; Thitiya Seesan; Prathan Buranasiri

doi:10.1117/12.2507775

4 March 2019 Improving qualitative phase imaging using error eliminating assist of two-steps phase shifting method in digital holography system

Chantira Boonsri, Thitiya Seesan, Prathan Buranasiri

Author Affiliations +

Proceedings Volume 10887, Quantitative Phase Imaging V; 108872G (2019) https://doi.org/10.1117/12.2507775
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

In this research, the mapping of the path length difference that involves the specimen is proposed for improving qualitative phase imaging (QPI) techniques. Phase-quality images are developed using the principle of phase- shift in digital holography and then this system has been applied to investigate biological cells and tissues. In our setup a microscopic digital holographic interferometer with polarizers and a quarter wave plate has been designed for detecting cells specimen. The resulting image contains information of the thickness in any area and the refractive index of the specimen. Moreover, a quadrature – phase shifting holography (QPSH) technique is purposed. Experimental results are shown improving QPI. Merits and limitations of this method are also described.

Citation Download Citation

Chantira Boonsri, Thitiya Seesan, and Prathan Buranasiri "Improving qualitative phase imaging using error eliminating assist of two-steps phase shifting method in digital holography system ", Proc. SPIE 10887, Quantitative Phase Imaging V, 108872G (4 March 2019); https://doi.org/10.1117/12.2507775

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