Motion-picture phase imaging by an integrated optical system of a parallel phase-shifting digital holographic microscope

Kazuki Shimizu; Takahito Fukuda; Tatsuya Hirakawa; Peng Xia; Yasuhiro Awatsuji; Kenzo Nishio; Osamu Matoba

doi:10.1117/12.2316616

24 April 2018 Motion-picture phase imaging by an integrated optical system of a parallel phase-shifting digital holographic microscope

Kazuki Shimizu, Takahito Fukuda, Tatsuya Hirakawa, Peng Xia, Yasuhiro Awatsuji, Kenzo Nishio, Osamu Matoba

Author Affiliations +

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 1071115 (2018) https://doi.org/10.1117/12.2316616
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

A parallel phase-shifting digital holographic microscope achieves motion-picture phase imaging of a dynamic minute specimen. However, much time and skill are required to construct the optical system of the microscope. The authors designed and constructed an optical system of a parallel phase-shifting digital holographic microscope in which the optical components of the microscope were integrated on a breadboard standing perpendicular to an optical table. The lateral and longitudinal magnifications of the microscope are 10 and 100, respectively. The authors experimentally demonstrated the motion-picture phase imaging of a dynamic minute specimen by the microscope. The holograms were recorded at 1,000 fps and the shutter speed was 0.5 ms.

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Kazuki Shimizu, Takahito Fukuda, Tatsuya Hirakawa, Peng Xia, Yasuhiro Awatsuji, Kenzo Nishio, and Osamu Matoba "Motion-picture phase imaging by an integrated optical system of a parallel phase-shifting digital holographic microscope", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 1071115 (24 April 2018); https://doi.org/10.1117/12.2316616

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