Single-exposure label-free multi-wavelength quantitative phase imaging using spatial multiplexing (Conference Presentation)

Nir A. Turko; Natan T. Shaked

doi:10.1117/12.2506100

4 March 2019 Single-exposure label-free multi-wavelength quantitative phase imaging using spatial multiplexing (Conference Presentation)

Nir A. Turko, Natan T. Shaked

Author Affiliations +

Proceedings Volume 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019; 1089022 (2019) https://doi.org/10.1117/12.2506100
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

We present our latest advances in multi-wavelength label-free imaging using quantitative phase imaging. Basic QPI designs usually incorporate a single, coherent or partially coherent light source. The introduction of off-axis geometry allows for calculation of phase information from a single-exposure interferogram. For multi-wavelength imaging, it has been previously required to take sequential interferograms with different wavelengths, effectively reducing imaging throughput, or to use color cameras that have cross-talk between the color channels and artefacts caused by Bayer pattern. Alternatively, it was suggested to use spatial interferometric multiplexing in order to provide multi-wavelength data, where multiple interferograms of different off-axis angles are acquired in a single exposure. We introduce an external module for label-free QPI capable of multi-color imaging using spatial interferometric multiplexing of multiple color channels, with self-referencing that significantly reduces mechanical vibration noise. This technique has various applications such as interferometric spectroscopy of live cells and multiple wavelength phase unwrapping.

Conference Presentation

Citation Download Citation

Nir A. Turko and Natan T. Shaked "Single-exposure label-free multi-wavelength quantitative phase imaging using spatial multiplexing (Conference Presentation)", Proc. SPIE 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019, 1089022 (4 March 2019); https://doi.org/10.1117/12.2506100

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KEYWORDS

Multiplexing

Phase imaging

Interferometry

Cameras

Data acquisition

Light sources

Spectroscopy

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