Metasurface-enhanced infrared spectroscopy for continuously monitoring the effect of cholesterol depletion in live cells

Steven H. Huang; Robert Delgado; Gennady B. Shvets

doi:10.1117/12.2547141

21 February 2020 Metasurface-enhanced infrared spectroscopy for continuously monitoring the effect of cholesterol depletion in live cells

Steven H. Huang, Robert Delgado, Gennady B. Shvets

Author Affiliations +

Proceedings Volume 11236, Biomedical Vibrational Spectroscopy 2020: Advances in Research and Industry; 112360P (2020) https://doi.org/10.1117/12.2547141
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Surface-enhanced infrared absorption (SEIRA) based on top-down fabricated nanostructures such as nanoantennas and metasurfaces has attracted much attention in recent years. These plasmonic resonant nanostructures can enhance the IR absorption signal of nearby molecules through its nearfield enhancement and have been shown to be able to detect adsorbed monolayers of proteins and lipids through their IR absorption spectra. Here, we demonstrate the continuous monitoring of cellular responses to stimuli using metasurface-enhanced infrared spectroscopy (MEIRS). A431 cells are seeded on a gold plasmonic metasurface fabricated on CaF₂ substrate. Continuous monitoring is made possible by integrating the metasurface with a flow chamber, and the IR absorption spectra of the attached cells are measured in reflectance mode under continuous perfusion of cell culture medium. Scanning electron microscopy (SEM) revealed that the cells preferentially adhere to gold surfaces rather than CaF₂ surfaces, suggesting that the IR signal measured through MEIRS is highly sensitive to the cells’ attachment and interaction with the gold metasurface. We have monitored the effect of methyl-beta-cyclodextrin, a cholesterol-depleting compound, on A431 cells. Principal component analysis highlighted the complex and subtle spectral changes of the cells.

Conference Presentation

Citation Download Citation

Steven H. Huang, Robert Delgado, and Gennady B. Shvets "Metasurface-enhanced infrared spectroscopy for continuously monitoring the effect of cholesterol depletion in live cells", Proc. SPIE 11236, Biomedical Vibrational Spectroscopy 2020: Advances in Research and Industry, 112360P (21 February 2020); https://doi.org/10.1117/12.2547141

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