Large volumetric optical-resolution photoacoustic microscopy based on contourlet transform fusion

Xianlin Song; Jianshuang Wei; Lingfang Song

doi:10.1117/12.2581366

8 November 2020 Large volumetric optical-resolution photoacoustic microscopy based on contourlet transform fusion

Xianlin Song, Jianshuang Wei, Lingfang Song

Proceedings Volume 11525, SPIE Future Sensing Technologies; 115252F (2020) https://doi.org/10.1117/12.2581366
Event: SPIE Future Sensing Technologies, 2020, Online Only

Conference Poster

Abstract

Photoacoustic imaging is a promising technique that combines optical contrast with ultrasonic detection to map the distribution of the absorbing pigments in biological tissues. Photoacoustic microscopy with large depth of focus is significant to the biomedical research. The conventional optical-resolution photoacoustic microscope (OR-PAM) suffers from limited depth of field (DoF) since the employed focused Gaussian beam only has a narrow depth range in focus, little details in depth direction can be revealed. Here, we developed a large volumetric optical-resolution photoacoustic microscopy based on contourlet transform fusion rules. Using a self-made optical-resolution photoacoustic microscope to obtain source images of the same sample with different focus. Firstly, the contourlet transform is used to decompose the source images A and B in J-layers to obtain subband coefficients of different scales and directions. Secondly, The PCA fusion rule is used for the low-pass subband coefficients of the source images A and B, and the SSIM fusion rule is used for the bandpass direction subband coefficients to obtain the contourlet coefficient of the fused image. Finally, performing contourlet inverse transformation on the fused coefficients to produce the final fused image. Simulation was performed to test the performance of our method, different focused images were used to verify the feasibility of the method. Performance of our method was analyzed by calculating Entropy, Average gradient, Mean Square Error (MSE) and Edge strength. The result of simulation shown that this method can extend the depth of field of PAM two times without the sacrifice of lateral resolution. And the in vivo imaging of zebra fish further demonstrates the feasibility of our method.

Citation Download Citation

Xianlin Song, Jianshuang Wei, and Lingfang Song "Large volumetric optical-resolution photoacoustic microscopy based on contourlet transform fusion", Proc. SPIE 11525, SPIE Future Sensing Technologies, 115252F (8 November 2020); https://doi.org/10.1117/12.2581366

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