Synthetic large volumetric optical-resolution photoacoustic microscopy using morphological pyramid fusion

Xianlin Song; Jianshuang Wei; Lingfang Song

doi:10.1117/12.2581370

5 March 2021 Synthetic large volumetric optical-resolution photoacoustic microscopy using morphological pyramid fusion

Xianlin Song, Jianshuang Wei, Lingfang Song

Proceedings Volume 11634, Multimodal Biomedical Imaging XVI; 116340T (2021) https://doi.org/10.1117/12.2581370
Event: SPIE BiOS, 2021, Online Only

Conference Poster

Abstract

Photoacoustic imaging is a promising technique that combines optical contrast with ultrasonic detection to map the distribution of the absorbing pigments in biological tissues. Photoacoustic microscopy with large depth of focus is significant to the biomedical research. The conventional optical-resolution photoacoustic microscope (OR-PAM) suffers from limited depth of field (DoF) since the employed focused Gaussian beam only has a narrow depth range in focus, little details in depth direction can be revealed. Here, we developed a synthetic large volumetric optical-resolution photoacoustic microscopy using morphological pyramid fusion. A self-made optical-resolution photoacoustic microscope was used to obtain source images of the same sample with different focus. Firstly, morphological smoothing was performed on the source images, then source images were decomposed using morphological pyramid, and finally the fusion image was obtained by performing inverse morphological pyramid transform. Simulation was performed to test the performance of our method, different focused images were used to verify the feasibility of the method. Performance of our method was analyzed by calculating Entropy, Average gradient, Mean Square Error (MSE) and Edge strength. The result of simulation shown that this method can extend the depth of field of PAM two times without the sacrifice of lateral resolution. And the in vivo imaging of the mouse cerebral vasculature with intact skull further demonstrates the feasibility of our method.

Citation Download Citation

Xianlin Song, Jianshuang Wei, and Lingfang Song "Synthetic large volumetric optical-resolution photoacoustic microscopy using morphological pyramid fusion", Proc. SPIE 11634, Multimodal Biomedical Imaging XVI, 116340T (5 March 2021); https://doi.org/10.1117/12.2581370

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