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5 March 2021 Holography cytology for imaging cellular biomechanics
Adam P. Wax
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Proceedings Volume 11645, Optical Elastography and Tissue Biomechanics VIII; 116450B (2021) https://doi.org/10.1117/12.2578682
Event: SPIE BiOS, 2021, Online Only
Abstract
Cell response to mechanical stimuli are essential for cell function yet not well understood. Existing rheological tools typically have limited throughput or require complex schemes. We have developed quantitative phase imaging methods which can image cell structural changes due to mechanical stimuli at the nanoscale. These methods are label free and can image cells in culture or flowing through microfluidic chips, providing high throughput measurements. We will present our holographic cytology imaging system and show its application to profiling large samples of red blood cells. Studies of cancer cells will show that refractive index variance is linked to measured cellular mechanical properties such as shear modulus. Additional applications include examining modulation of mechanical properties due to early carcinogenic events, investigating the role of specific cellular structural proteins in mechanotransduction and tracking water and ion channel regulation due to mechanical stress.
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Adam P. Wax "Holography cytology for imaging cellular biomechanics", Proc. SPIE 11645, Optical Elastography and Tissue Biomechanics VIII, 116450B (5 March 2021); https://doi.org/10.1117/12.2578682
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KEYWORDS
Cell biology
Holography
Imaging systems
Ion channels
Microfluidics
Modulation
Phase imaging
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