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5 March 2021 Three-dimensional super-resolution microscopy in thicker specimens using adaptive optics
Martin J. Booth
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Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 1164908 (2021) https://doi.org/10.1117/12.2585296
Event: SPIE BiOS, 2021, Online Only
Abstract
Adaptive optics (AO) is used to correct aberrations when focussing deep into specimens and is particularly important in super-resolution nanoscopy methods, which are particularly sensitive. Novel AO methods have been developed to deal with the particular challenges posed by super-resolution microscopes. We show how various three-dimensional nanoscopy applications can be facilitated using AO. Specifically, this includes whole cell and tissue imaging using a 4Pi single molecule localisation microscope, which uses dual opposing objective lenses and two deformable mirrors for improved z resolution; structured illumination microscopy, with adaptive illumination and aberration correction; and STED-fluorescence correlation spectroscopy in living cells.
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Martin J. Booth "Three-dimensional super-resolution microscopy in thicker specimens using adaptive optics", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164908 (5 March 2021); https://doi.org/10.1117/12.2585296
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KEYWORDS
Adaptive optics
Super resolution microscopy
Aberration correction
Fluorescence correlation spectroscopy
Microscopes
Microscopy
Super resolution
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