Six-pack holographic imaging for dynamic rejection of out-of-focus objects

Simcha K. Mirsky; Natan T. Shaked

doi:10.1117/12.2582691

5 March 2021 Six-pack holographic imaging for dynamic rejection of out-of-focus objects

Simcha K. Mirsky, Natan T. Shaked

Proceedings Volume 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021; 116550Y (2021) https://doi.org/10.1117/12.2582691
Event: SPIE BiOS, 2021, Online Only

Abstract

Six-pack holography may be used to reject out-of-focus objects in dynamic samples. By illuminating the sample from six different angles in parallel, out-of-focus objects are laterally shifted in six different directions when projected onto the focal plane. Pixel-wise averaging of the six reconstructed images then reduces the impact of out-of-focus objects. The averaged images reduce the contribution of out-of-focus objects by up to 83%, depending on the out-of-focus object axial distance from the focused plane. Simulation results are shown, as well as experimental results for swimming microalgae and flowing microbeads, including numerical refocusing by Fresnel propagation.

Conference Presentation

Citation Download Citation

Simcha K. Mirsky and Natan T. Shaked "Six-pack holographic imaging for dynamic rejection of out-of-focus objects", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116550Y (5 March 2021); https://doi.org/10.1117/12.2582691

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