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20 June 2021 Quantitative analysis of malignant and normal bladder tissue based on combination of interferometric phase microscopy and Raman spectroscopy
Almog Taieb, Garry Berkovic, Miki Haifler, Natan T. Shaked
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Proceedings Volume 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V; 1178618 (2021) https://doi.org/10.1117/12.2593397
Event: SPIE Optical Metrology, 2021, Online Only
Abstract
We propose a multimodal quantitative, label-free and nondestructive diagnostic metrology technique by integrating off-axis interferometric phase microscopy (IPM) and Raman spectroscopy (RS), for analyzing normal and malignant bladder tissue samples. We built a Mach–Zehnder interferometer connected to a commercial confocal microscope for imaging a large area of tissue slices, up to a few millimeters, by semi-automatic scanning of the tissue. Bright-field image of hematoxylin and eosin stained tissue slice of the same area was also acquired. Measurements of Raman spectra were acquired using our RS system with excitation wavelength of 561 nm. Using the quantitative phase information, we obtained various spatial and morphological parameters of the tissues such as the anisotropy factor, which demonstrated their direct correlation with tumor presence. This method is expected to be useful for stain-free cancer diagnosis, while obtaining both quantitative information about tissue morphological modifications and changes in tissue Raman scattering properties induced by cancer.
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Almog Taieb, Garry Berkovic, Miki Haifler, and Natan T. Shaked "Quantitative analysis of malignant and normal bladder tissue based on combination of interferometric phase microscopy and Raman spectroscopy", Proc. SPIE 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 1178618 (20 June 2021); https://doi.org/10.1117/12.2593397
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KEYWORDS
Raman spectroscopy
Tissues
Bladder
Interferometry
Microscopy
Phase interferometry
Tissue optics
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