Contrast and axial confinement enhancement in deep imaging via HiLo-based line-scanning temporal focusing microscopy

Ruheng Shi; Yuanlong Zhang; Tiankuang Zhou; Lingjie Kong

doi:10.1117/12.2615807

7 December 2021 Contrast and axial confinement enhancement in deep imaging via HiLo-based line-scanning temporal focusing microscopy

Ruheng Shi, Yuanlong Zhang, Tiankuang Zhou, Lingjie Kong

Author Affiliations +

Proceedings Volume 11922, Advances in Microscopic Imaging III; 119221B (2021) https://doi.org/10.1117/12.2615807
Event: European Conferences on Biomedical Optics, 2021, Online Only

Abstract

We propose HiLo based line-scanning temporal focusing microscopy to enhance contrast and axial confinement in deep imaging, and demonstrate its superiority by volumetric imaging of microglia and neurons in mouse brains in vivo.

Citation Download Citation

Ruheng Shi, Yuanlong Zhang, Tiankuang Zhou, and Lingjie Kong "Contrast and axial confinement enhancement in deep imaging via HiLo-based line-scanning temporal focusing microscopy", Proc. SPIE 11922, Advances in Microscopic Imaging III, 119221B (7 December 2021); https://doi.org/10.1117/12.2615807

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