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13 March 2024 Single-cell investigation of excitation wavefront propagation in in vitro human induced cardiomyocytes using a digital holographic stimulation system
Felix Schmieder, Lars Büttner, Adam T. Pierce, Wouter Derks, Olaf Bergmann, Jürgen W Czarske
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Proceedings Volume 12829, Optogenetics and Optical Manipulation 2024; 128290I (2024) https://doi.org/10.1117/12.3003492
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Cardiomyocytes form an electrically coupled syncytium, enabling synchronized action potential wavefronts. Optogenetics facilitates the study of cardiac excitation wavefronts in vivo and in vitro. We demonstrate a laser-optical system utilizing computer-generated holograms to stimulate and inhibit light-sensitive human stem-cell-derived cardiomyocytes, allowing e.g. non-destructive modeling of myocardial scarring and exctiation wavefront control. With rapid frame rates upt to 1.7 kHz, our system spatially positions multiple foci or complex illumination patterns, achieving defined 3D-distributed excitation wavefronts for quickly changing stimulation protocols. High-speed video microscopy assesses contractions in samples. We show successful coupling of cardiomyocyte contraction to optical stimulation and stimulation-wavefront shape-dependencies, paving the way for patient-specific disease modeling using multi-photong holographic light shaping and red-shifted opsins in 3D cardiac organoids.
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Felix Schmieder, Lars Büttner, Adam T. Pierce, Wouter Derks, Olaf Bergmann, and Jürgen W Czarske "Single-cell investigation of excitation wavefront propagation in in vitro human induced cardiomyocytes using a digital holographic stimulation system", Proc. SPIE 12829, Optogenetics and Optical Manipulation 2024, 128290I (13 March 2024); https://doi.org/10.1117/12.3003492
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KEYWORDS
Wavefronts
Digital holography
In vitro testing
Wave propagation
Holography
Action potentials
3D modeling
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