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Fs-LDW is a promising technique for fine 3D printing of biomaterials such as protein due to nonlinear multiphoton absorption processes facilitating localized microfabrication along a designated laser light path. The use of protein as a precursor material for fs-LDW is attractive because the fabricated structures retain their native functions as demonstrated by several reports. These reports range over various combination of protein and photoactivators, but pure protein can also be utilized as a precursor. The resulting proteinaceous microstructures with native function retained and submicron feature sizes might offer diverse biomedical or biochip applications. This work highlights the capabilities of fs-LDW from pure protein with a biologically highly regarded protein, broadening the scope of the 3D printing technology as well as providing a new use for the fluorescent protein family. |
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