Large-aperture CCD x-ray detector for protein crystallography using a fiber-optic taper

Michael G. Strauss; Edwin M. Westbrook; Istvan Naday; Tom A. Coleman; Mary L. Westbrook; Dale J. Travis; Robert M. Sweet; J. W. Pflugrath; Martin J. Stanton

doi:10.1117/12.45312

1 July 1991 Large-aperture CCD x-ray detector for protein crystallography using a fiber-optic taper

Michael G. Strauss, Edwin M. Westbrook, Istvan Naday, Tom A. Coleman, Mary L. Westbrook, Dale J. Travis, Robert M. Sweet, J. W. Pflugrath, Martin J. Stanton

Author Affiliations +

Proceedings Volume 1447, Charge-Coupled Devices and Solid State Optical Sensors II; (1991) https://doi.org/10.1117/12.45312
Event: Medical Imaging '91, 1991, San Jose, CA, United States

Abstract

A detector with a 114 mm aperture, based on a charge-coupled device (CCD), has been designed for x-ray diffraction studies in protein crystallography. The detector was tested on a beamline of the National Synchrotron Light Source at Brookhaven National Laboratory with a beam intensity greater than 109 x-ray photons/s. A fiber-optic taper, an image intensifier, and a lens demagnify, intensify, and focus the image onto a CCD having 512 X 512 pixels. A detective quantum efficiency (DQE) of 0.36 was obtained by evaluating the statistical uncertainty in the detector output. The dynamic range of a 4 X 4 pixel resolution element, comparable in size to a diffraction peak, was 10 4. The point-spread function shows FWHM resolution of approximately 1 pixel, where a pixel on the detector face is 160 micrometers . A complete data set, consisting of forty-five 1 degree(s) rotation frames, was obtained in just 36 s of x-ray exposure to a crystal of chicken egg-white lysozyme. In a separate experiment, a lysozyme data set consisting of 495 0.1 degree(s) frames, was processed by the MADNES data reduction program, yielding symmetry R-factors for the data of 3.2- 3.5. Diffraction images from crystals of the myosin S1 head (a equals 275 angstroms) were also recorded. The Bragg spots, only 5 pixels apart, were resolved but were not sufficiently separated to process these data. Changes in the detector design which will improve the DQE and spatial resolution are outlined. The overall performance showed that this type of detector is well suited for x-ray scattering investigations with synchrotron sources.

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Michael G. Strauss, Edwin M. Westbrook, Istvan Naday, Tom A. Coleman, Mary L. Westbrook, Dale J. Travis, Robert M. Sweet, J. W. Pflugrath, and Martin J. Stanton "Large-aperture CCD x-ray detector for protein crystallography using a fiber-optic taper", Proc. SPIE 1447, Charge-Coupled Devices and Solid State Optical Sensors II, (1 July 1991); https://doi.org/10.1117/12.45312

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