Analysis of excitation and collection geometries for planar waveguide immunosensors

Douglas A. Christensen; Shellee Dyer; David Fowers; James N. Herron

doi:10.1117/12.144833

21 May 1993 Analysis of excitation and collection geometries for planar waveguide immunosensors

Douglas A. Christensen, Shellee Dyer, David Fowers, James N. Herron

Proceedings Volume 1886, Fiber Optic Sensors in Medical Diagnostics; (1993) https://doi.org/10.1117/12.144833
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

We demonstrate the use of a two-channel flowcell for fluorescent immunoassays. The flowcell contains a planar silica waveguide for evanescent excitation of the fluorophores, and the planar waveguide surface provides the solid support for immobilization of the antibodies. The detection system is composed of a grating spectrometer and a CCD camera for spectral characterization of the emitted signals. Two methods of sensing have been studied: a displacement-type technique and a sandwich-type assay. The sensitivity achieved for measuring concentrations of HCG by the sandwich method is sub-picomolar. Also, we have experimentally compared the signal strengths for two alternative ways of excitation and collection, and determine that waveguide excitation/side collection has some practical advantages over side excitation/waveguide collection.

Citation Download Citation

Douglas A. Christensen, Shellee Dyer, David Fowers, and James N. Herron "Analysis of excitation and collection geometries for planar waveguide immunosensors", Proc. SPIE 1886, Fiber Optic Sensors in Medical Diagnostics, (21 May 1993); https://doi.org/10.1117/12.144833

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