Analysis of depth response for fluorescent confocal scanning microscope

ZhenSen Wu; Ping Zhang; Cuiying Wang

doi:10.1117/12.155731

3 September 1993 Analysis of depth response for fluorescent confocal scanning microscope

ZhenSen Wu, Ping Zhang, Cuiying Wang

Proceedings Volume 1889, Holography, Interferometry, and Optical Pattern Recognition in Biomedicine III; (1993) https://doi.org/10.1117/12.155731
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

A confocal scanning fluorescent microscope (FCSM) changes parallel imaging, which is usually done with a conventional fluorescent microscope, to series-imaging. Because of this unique feature of imaging mean, the FCSM has the longitudinal discrimination ability a conventional fluorescent microscope does not have. The imaging principles of a FCSM are described briefly. Through an established mathematical model of system transfer function, the depth resolutions are calculated numerically. In biology science, the aberrations are introduced when an oil-immersion objective is used to study thick specimens, such as tissues and living cells, whose reflective indexes are significantly different from that of the immersion oil used in the experiment. Finally, after numerical calculation, a set of optimization curves for compensating the aberrations are given graphically under the different conditions, the compensation method proposed in this paper is suitable both for oil-immersion and non- immersion objectives.

Citation Download Citation

ZhenSen Wu, Ping Zhang, and Cuiying Wang "Analysis of depth response for fluorescent confocal scanning microscope", Proc. SPIE 1889, Holography, Interferometry, and Optical Pattern Recognition in Biomedicine III, (3 September 1993); https://doi.org/10.1117/12.155731

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Microscopes

Objectives

Confocal microscopy

Refractive index

Luminescence

Sensors

Lens design

Show All Keywords

Keywords/Phrases

Search In:

Publication Years