Inhibition of vascular smooth muscle cells by photodynamic therapy is effective and depends on intracellular Photofrin

Mohammed S. Sobeh; Philip Chan; Robert J. Ham; Frank W. Cross M.D.

doi:10.1117/12.179928

12 July 1994 Inhibition of vascular smooth muscle cells by photodynamic therapy is effective and depends on intracellular Photofrin

Mohammed S. Sobeh, Philip Chan, Robert J. Ham, Frank W. Cross M.D.

Author Affiliations +

Proceedings Volume 2130, Diagnostic and Therapeutic Cardiovascular Interventions IV; (1994) https://doi.org/10.1117/12.179928
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Photodynamic therapy (PDT) is a promising treatment for vascular restenosis. Determining the site of the photosensitiser responsible for cytotoxicity during PDT is essential for planning treatment strategies. Vascular smooth muscle cells (VSMCs) were cultured from 6 individuals using substrate attached explant techniques and transferred into microtitire plates at 50,000 cells/well. Cells were arranged in 3 groups; the first group was incubated with 2 (mu) gml^-1 of Photofrin for 48 hours, followed by polychromatic light illumination of 3 Jcm^-2. In the second group the extracellular Photofrin was removed by washing cells just prior to illumination, and in the third group Photofrin was only added immediately prior to illumination. Results are expressed as mean percentage cell survival+/- SEM. Cells were minimally affected by extracellular Photofrin alone (91.9+/- 10.7). Photofrin in the intracellular and (intra+extracellular) compartments reduced VSMCs survival to (7.3+/- 4.9) and (8.8+/- 2.6). The mechanism of ablation appears to be due to activation of the intracellular rather than residual Photofrin in the extracellular medium

Citation Download Citation

Mohammed S. Sobeh, Philip Chan, Robert J. Ham, and Frank W. Cross M.D. "Inhibition of vascular smooth muscle cells by photodynamic therapy is effective and depends on intracellular Photofrin", Proc. SPIE 2130, Diagnostic and Therapeutic Cardiovascular Interventions IV, (12 July 1994); https://doi.org/10.1117/12.179928

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available