Light-scattering analysis of ultrasonic wave's influence on the RBC agglutination in vitro

Valeri A. Doubrovski; Costanten N. Dvoretski

doi:10.1117/12.347492

30 April 1999 Light-scattering analysis of ultrasonic wave's influence on the RBC agglutination in vitro

Valeri A. Doubrovski, Costanten N. Dvoretski

Proceedings Volume 3598, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications III; (1999) https://doi.org/10.1117/12.347492
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Elastic light scattering is one of the most often used optical methods to analyze the cells agglutination reaction - the base of a great number of medical diagnostic test and biomedical investigations. The increase of the resolution of methods and apparatus towards the induced cells aggregation - the foundation of the reaction of agglutination, is quite an actual problem. The solution of this problem increases the reliability of the diagnostic test and gives an opportunity to achieve the diagnostic information in the cases when the traditional approaches do not lead to the diagnostic results. The attempt to increase the resolution of the immune reaction analyzer by means of ultrasonic waves action on the reagent mixture in vitro is taken in this paper. The RBC agglutination reaction which is usually used for the blood group type examination is chosen as an example of an object of the investigation. Different laser optical trains of the devices based on the turbidimetric and nephelometric methods and their combination are analyzed here. The influence of the ultrasonic wave time interval action and of the features of the sample preparation procedure on the resolution towards the agglutination process was investigated in this work. It is shown that the ultrasonic wave action on the reagent mixture leads to a large gain in the resolution of the device towards the RBC agglutination process. The experiments showed that the resolution of the device was enough to register the agglutination process even for the erythrocytes with weak agglutination ability when the reaction was invisible without ultrasonic action. It occurred that the diagnostic test time was more than by an order shortened due to the ultrasonic wave action. The optimal ultrasonic time interval action, the sample preparation technology and experimental technique were defined. The principle of the ultrasonic wave action on the cells agglutination process suggested here can be spread out on the immune molecular media. The results may be useful to develop new apparatus and methods for the aims of medical laboratory diagnostics.

Citation Download Citation

Valeri A. Doubrovski and Costanten N. Dvoretski "Light-scattering analysis of ultrasonic wave's influence on the RBC agglutination in vitro", Proc. SPIE 3598, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications III, (30 April 1999); https://doi.org/10.1117/12.347492

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KEYWORDS

Ultrasonics

Blood

Channel projecting optics

Light scattering

Biomedical optics

Diagnostic tests

Diagnostics

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