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The main idea of the present work is to combine in the same film different enzymes and pH sensitive organic dyes in order to form an optical transducer. Decomposition of substrate molecules, catalyzed by enzymes, usually accompanied by pH changes in local surrounding, which can be registered by spectral transformations of indicator molecules. This idea was realized by using cyclo- tetrachromotropylene (Chromo1) as an indicator and an enzyme, such as Urease.
Alexei V. Nabok,Asim K. Ray,Aseel K. Hassan,Robert B. Yates, andRui Majeed
"Composite polyelectrolyte self-assembled films for optical biosensors", Proc. SPIE 3673, Smart Structures and Materials 1999: Smart Electronics and MEMS, (20 July 1999); https://doi.org/10.1117/12.354273
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Alexei V. Nabok, Asim K. Ray, Aseel K. Hassan, Robert B. Yates, Rui Majeed, "Composite polyelectrolyte self-assembled films for optical biosensors," Proc. SPIE 3673, Smart Structures and Materials 1999: Smart Electronics and MEMS, (20 July 1999); https://doi.org/10.1117/12.354273