Pigmentation in NIR laser tissue damage

Michael L. Denton; Debbie M. Eikum; Gary D. Noojin; David J. Stolarski; Robert J. Thomas; Randolph D. Glickman; Benjamin A. Rockwell

doi:10.1117/12.476898

20 June 2003 Pigmentation in NIR laser tissue damage

Michael L. Denton, Debbie M. Eikum, Gary D. Noojin, David J. Stolarski, Robert J. Thomas, Randolph D. Glickman, Benjamin A. Rockwell

Author Affiliations +

Proceedings Volume 4953, Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment III; (2003) https://doi.org/10.1117/12.476898
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Damage from femtosecond mode-locked laser exposure (total exposure times longer than 20 microseconds) is predicted to be mediated by the same thermal mechanism found for continuous wave (CW) laser exposure. Experimentally, this trend holds true when comparing minimum visible lesions (MVL) from 0.25 s mode-locked and CW near-IR exposures in vivo. To further test this hypothesis in an in vitro setting we compared threshold values for cell death at various times post-exposure for mode-locked and CW 810-nm laser exposures. Using an artificially pigmented RPE cell culture system we show that damaging absorption of NIR laser light is directly related to the presence of melanin pigment granules, and that the damage thresholds for mode-locked and CW laser exposures are essentially the same for the pigmented cells. We additionally conclude that the artificially pigmented RPE cell cultures are a reasonable model system for the study of thermal and photomechanical forms of ocular laser tissue damage.

Citation Download Citation

Michael L. Denton, Debbie M. Eikum, Gary D. Noojin, David J. Stolarski, Robert J. Thomas, Randolph D. Glickman, and Benjamin A. Rockwell "Pigmentation in NIR laser tissue damage", Proc. SPIE 4953, Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment III, (20 June 2003); https://doi.org/10.1117/12.476898

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