Toward a disposable real-time DNA biosensing platform

Layne D. Williams; Justin Bishop; Steve Blair; David W. Peters; Murat Okandan

doi:10.1117/12.524629

23 December 2003 Toward a disposable real-time DNA biosensing platform

Layne D. Williams, Justin Bishop, Steve Blair, David W. Peters, Murat Okandan

Proceedings Volume 5345, Microfluidics, BioMEMS, and Medical Microsystems II; (2003) https://doi.org/10.1117/12.524629
Event: Micromachining and Microfabrication, 2004, San Jose, California, United States

Abstract

With the goal of a portable diagnostic system in mind, we have designed a disposable platform for DNA detection. Surface micromachining using the SwIFT process at Sandia National Laboratories was used to make the new device, combining a waveguide, grating optics, heating structures, on-chip pumping, and microfluidics in a disposable package. PDMS microfluidic channels are integrated with the surface micromachined device to enable higher flow rates and added fluid complexity. Work on DNA hybridization under flow is presented, as applies to the function of the sensor. A description of the platform covering heating of the waveguide surface, laser coupling into the waveguide using grating optics, attachment chemistry for the sensor surface, and sealing of the PDMS microfluidic system to the device is given.

Citation Download Citation

Layne D. Williams, Justin Bishop, Steve Blair, David W. Peters, and Murat Okandan "Toward a disposable real-time DNA biosensing platform", Proc. SPIE 5345, Microfluidics, BioMEMS, and Medical Microsystems II, (23 December 2003); https://doi.org/10.1117/12.524629

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