Influence of laser irradiation on superoxide dismutase activity of human lymphocytes in vitro

Nataly M. Kozlova; Anna N. Antonovich; Ekaterina I. Slobozhanina

doi:10.1117/12.753428

1 August 2007 Influence of laser irradiation on superoxide dismutase activity of human lymphocytes in vitro

Nataly M. Kozlova, Anna N. Antonovich, Ekaterina I. Slobozhanina

Proceedings Volume 6734, International Conference on Lasers, Applications, and Technologies 2007: Laser Technologies for Medicine; 67341R (2007) https://doi.org/10.1117/12.753428
Event: International Conference on Lasers, Applications, and Technologies '07, 2007, Minsk, Belarus

Abstract

The treatment of blood with low intensity laser irradiation has become popular in a variety of clinical applications due to its anti-inflammatory, biostimulative and immune-stimulatory effects etc. Laser blood irradiation with infrared and red laser sources have the potential for stimulating antioxidant enzymes activities. At present study the influence of red and infra-red laser irradiation at different doses on superoxide dismutase (SOD) activity of peripheral blood lymphocytes was investigated in vitro. Suspensions of human lymphocytes (concentration of cells 1x10⁶ cells/ml) were irradiated with red (670 nm) and infrared (980 nm) therapy lasers at different light doses (0-600 J/sample) and light power (4,5 and 15 mW for red; 50 and 500 mW for infrared) at 20°C. It is revealed doze-depended effect of red and infra-red laser irradiation on superoxide dismutaze activity of peripheral blood lymphocytes. The SOD activity, first of all, depends on irradiation time, but not on intensity or wavelength of irradiation. These data can explain the positive medical effects of a laser blood irradiation. The obtained results confirm a hypothesis that laser irradiation with the different wavelength characteristic (red and infra-red light ranges) reveals a stimulating effect on SOD - antioxidant defence system enzyme in peripheral blood lymphocytes.

Citation Download Citation

Nataly M. Kozlova, Anna N. Antonovich, and Ekaterina I. Slobozhanina "Influence of laser irradiation on superoxide dismutase activity of human lymphocytes in vitro", Proc. SPIE 6734, International Conference on Lasers, Applications, and Technologies 2007: Laser Technologies for Medicine, 67341R (1 August 2007); https://doi.org/10.1117/12.753428

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KEYWORDS

Blood

Laser irradiation

Laser therapeutics

In vitro testing

Infrared radiation

Tissues

Defense systems

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