Fabrication of gold dots for DNA based nano-sensors

Mashiur Rahman; B. Scott Day; Huan Cao; Aaron Gin; Michael L. Norton

doi:10.1117/12.734487

11 October 2007 Fabrication of gold dots for DNA based nano-sensors

Mashiur Rahman, B. Scott Day, Huan Cao, Aaron Gin, Michael L. Norton

Proceedings Volume 6769, Nanosensing: Materials, Devices, and Systems III; 67690H (2007) https://doi.org/10.1117/12.734487
Event: Optics East, 2007, Boston, MA, United States

Abstract

Here we report efforts towards fabrication of DNA based nanosensors, where DNA molecules were immobilized on gold-dot arrays. AFM was used as the main characterization tool. Two major challenges associated with characterizing these nanosensors are: 1) constructing small (<10 nm high) attachment sites on a flat surface to resolve the details of the hybridized DNA lattice and 2) fabricating attachment sites on a surface that allows the lattices to be well separated. We have chosen silicon as the substrate since atomically flat surfaces are readily available. For the formation of the gold-dot arrays, we combined e-beam lithography with metal deposition via an e-beam evaporation. The fabrication process was confirmed by AFM imaging. For DNA attachment we have used DNA functionalized on one end with multi-thiolated dendrimers, an attachment strategy developed in our lab. After exposing the surface to the DNA solution, the DNA was found to be attached to ~5% of the gold dots. To improve the attachment technique, oxygen plasma cleaning and ethanol treatment have been investigated. Finally, a higher yield (65% of total scanned areas) of DNA attachment to the gold dots was achieved.

Citation Download Citation

Mashiur Rahman, B. Scott Day, Huan Cao, Aaron Gin, and Michael L. Norton "Fabrication of gold dots for DNA based nano-sensors", Proc. SPIE 6769, Nanosensing: Materials, Devices, and Systems III, 67690H (11 October 2007); https://doi.org/10.1117/12.734487

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