Bio-functional Au/Si nanorods for pathogen detection

Bosoon Park; Junxue Fu; Yiping Zhao; Gregory R. Siragusa; Yong-Jin Cho; Kurt C. Lawrence; William R. Windham

doi:10.1117/12.736486

25 September 2007 Bio-functional Au/Si nanorods for pathogen detection

Bosoon Park, Junxue Fu, Yiping Zhao, Gregory R. Siragusa, Yong-Jin Cho, Kurt C. Lawrence, William R. Windham

Proceedings Volume 6769, Nanosensing: Materials, Devices, and Systems III; 67690O (2007) https://doi.org/10.1117/12.736486
Event: Optics East, 2007, Boston, MA, United States

Abstract

Nanotechnology applications for food safety and biosecurity, especially development of nanoscale sensors for foodborne pathogen measurement are emerging. A novel bio-functional nanosensor for Salmonella detection was developed using hetero-nanorods. The silica nanorods were fabricated by glancing angle deposition method and the gold was sputtered onto the silica nanorods. Alexa488-succinimide dye was immobilized onto the annealed Si nanorods via the attachment between dye ester and primary amine group supplied by the 3-Aminopropyltriethoxysilane. The anti-Salmonella was conjugated to gold via Dithiobis[succinimidylpropionate] self-assembly monolayer. Due to the high aspect ratio nature of the Si nanorods, hundreds or thousands of dye molecules attached to the Si nanorods produced enhanced fluorescence signal. These biologically functionalized nanorods can be used to detect Salmonella with fluorescent microscopic imaging. This new nanoscale biosensor will be able to detect other foodborne pathogenic bacteria for food safety and security applications.

Citation Download Citation

Bosoon Park, Junxue Fu, Yiping Zhao, Gregory R. Siragusa, Yong-Jin Cho, Kurt C. Lawrence, and William R. Windham "Bio-functional Au/Si nanorods for pathogen detection", Proc. SPIE 6769, Nanosensing: Materials, Devices, and Systems III, 67690O (25 September 2007); https://doi.org/10.1117/12.736486

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