Nano-aquarium with microfluidic structures fabricated by femtosecond laser direct writing for dynamic microorganism analysis

Y. Hanada; K. Sugioka; H. Kawano; I. Ishikawa; A. Miyawaki; K. Midorikawa

doi:10.1117/12.823397

18 February 2009 Nano-aquarium with microfluidic structures fabricated by femtosecond laser direct writing for dynamic microorganism analysis

Y. Hanada, K. Sugioka, H. Kawano, I. Ishikawa, A. Miyawaki, K. Midorikawa

Author Affiliations +

Proceedings Volume 7276, Photonics and Optoelectronics Meetings (POEM) 2008: Laser Technology and Applications; 72760Z (2009) https://doi.org/10.1117/12.823397
Event: Photonics and Optoelectronics Meetings, 2008, Wuhan, China

Abstract

We demonstrate the fabrication of three-dimensional (3D) hollow microstructures embedded in photostructurable glass by a nonlinear multiphoton absorption process using a femtosecond (fs) laser. Fs laser direct writing followed by annealing and successive wet etching in dilute hydrofluoric (HF) acid solution resulted in the rapid manufacturing of microchips with 3-D hollow microstructures for the dynamic observation of living microorganisms in fresh water. The embedded microchannel structure enables us to analyze the continuous motion of Euglena gracilis and Cryptomonas. Such microchips, referred to as nano-aquariums realize the efficient and highly functional observation of microorganisms.

Citation Download Citation

Y. Hanada, K. Sugioka, H. Kawano, I. Ishikawa, A. Miyawaki, and K. Midorikawa "Nano-aquarium with microfluidic structures fabricated by femtosecond laser direct writing for dynamic microorganism analysis", Proc. SPIE 7276, Photonics and Optoelectronics Meetings (POEM) 2008: Laser Technology and Applications, 72760Z (18 February 2009); https://doi.org/10.1117/12.823397

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