Measurement of light scattering from trapped particles

Mikael Collins; Antti Kauppila; Artashes Karmenyan; Lech Gajewski; Krzysztof Szewczyk; Matti Kinnunen; Risto Myllylä

doi:10.1117/12.871613

24 November 2010 Measurement of light scattering from trapped particles

Mikael Collins, Antti Kauppila, Artashes Karmenyan, Lech Gajewski, Krzysztof Szewczyk, Matti Kinnunen, Risto Myllylä

Proceedings Volume 7376, Laser Applications in Life Sciences; 737619 (2010) https://doi.org/10.1117/12.871613
Event: Laser Applications in Life Sciences 2010, 2010, Oulu, Finland

Abstract

We have developed an optical tweezers setup combined with a laser light scattering measurement system to measure the elastic light scattering from trapped particles. The setup consists of a near infrared laser (λ=1064nm), a water immersion objective for trapping, a single or double structure sample cuvette, and a HeNe-laser for illuminating the trapped particles. The light is detected with an amplified photomultiplier and a lock-in amplifier. Light scattering images from the trapped particles are also shown with a CCD camera. An optical trap keeps the particle stable during the measurement. The measured scattering patterns from 23.25 μm diameter polystyrene spheres were shown to have good comparability with theoretical modelling. 6.0 μm particles were also measured. The light scattering from trapped red blood cells was much weaker than that from 23.25 μm and 6.0 μm polystyrene spheres, almost at the detection limit of our current detection system configuration. The stability of the polystyrene sphere was much better during the measurements than that of the red blood cell.

Citation Download Citation

Mikael Collins, Antti Kauppila, Artashes Karmenyan, Lech Gajewski, Krzysztof Szewczyk, Matti Kinnunen, and Risto Myllylä "Measurement of light scattering from trapped particles", Proc. SPIE 7376, Laser Applications in Life Sciences, 737619 (24 November 2010); https://doi.org/10.1117/12.871613

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