Confocal imaging at the nanoscale with two-color STED microscopy

Hilmar Gugel; Arnold Giske; Marcus Dyba; Jochen Sieber

doi:10.1117/12.874779

28 February 2011 Confocal imaging at the nanoscale with two-color STED microscopy

Hilmar Gugel, Arnold Giske, Marcus Dyba, Jochen Sieber

Proceedings Volume 7905, Single Molecule Spectroscopy and Imaging IV; 79050X (2011) https://doi.org/10.1117/12.874779
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

STED microscopy enables confocal imaging of biological samples with a resolution that is not limited by diffraction. It provides new insights in various fields of biology, such as membrane biology, neurobiology and physiology. Its three dimensional sectioning ability allows the acquisition of high resolution image stacks. Furthermore, STED microscopy enables the recording of dynamic processes and live cell images. We present two-color imaging in confocal STED microscopy with a single STED wavelength. Pulsed and continuous wave lasers in the visible and near infra-red wavelengths range are used for stimulated emission. The resolution enhancement is demonstrated in comparison to confocal imaging with biological specimens.

Citation Download Citation

Hilmar Gugel, Arnold Giske, Marcus Dyba, and Jochen Sieber "Confocal imaging at the nanoscale with two-color STED microscopy", Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050X (28 February 2011); https://doi.org/10.1117/12.874779

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