Non-invasive imaging of skin cancer with fluorescence lifetime imaging using two photon tomography

Rakesh Patalay; Clifford Talbot; Yuriy Alexandrov; Ian Munro; Hans Georg Breunig; Karsten König; Sean Warren; Mark A. A. Neil; Paul M. W. French; Anthony Chu; Gordon W. Stamp; Christopher Dunsby

doi:10.1117/12.889314

14 June 2011 Non-invasive imaging of skin cancer with fluorescence lifetime imaging using two photon tomography

Rakesh Patalay, Clifford Talbot, Yuriy Alexandrov, Ian Munro, Hans Georg Breunig, Karsten König, Sean Warren, Mark A. A. Neil, Paul M. W. French, Anthony Chu, Gordon W. Stamp, Christopher Dunsby

Author Affiliations +

Proceedings Volume 8087, Clinical and Biomedical Spectroscopy and Imaging II; 808718 (2011) https://doi.org/10.1117/12.889314
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

Multispectral fluorescence lifetime imaging (FLIM) using two photon microscopy as a non-invasive technique for the diagnosis of skin lesions is described. Skin contains fluorophores including elastin, keratin, collagen, FAD and NADH. This endogenous contrast allows tissue to be imaged without the addition of exogenous agents and allows the in vivo state of cells and tissues to be studied. A modified DermaInspect® multiphoton tomography system was used to excite autofluorescence at 760 nm in vivo and on freshly excised ex vivo tissue. This instrument simultaneously acquires fluorescence lifetime images in four spectral channels between 360-655 nm using time-correlated single photon counting and can also provide hyperspectral images. The multispectral fluorescence lifetime images were spatially segmented and binned to determine lifetimes for each cell by fitting to a double exponential lifetime model. A comparative analysis between the cellular lifetimes from different diagnoses demonstrates significant diagnostic potential.

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Rakesh Patalay, Clifford Talbot, Yuriy Alexandrov, Ian Munro, Hans Georg Breunig, Karsten König, Sean Warren, Mark A. A. Neil, Paul M. W. French, Anthony Chu, Gordon W. Stamp, and Christopher Dunsby "Non-invasive imaging of skin cancer with fluorescence lifetime imaging using two photon tomography", Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 808718 (14 June 2011); https://doi.org/10.1117/12.889314

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