A study of a self diagnostic platform for the detection of A2 biomarker for Leishmania donovani

Philip J. R. Roche; Maurice C-K. Cheung; Mohamed Najih; Laura-Isobel McCall; Ibrahim Fakih; Vamsy P. Chodavarapu; Brian Ward; Momar Ndao; Andrew G. Kirk

doi:10.1117/12.910146

14 February 2012 A study of a self diagnostic platform for the detection of A2 biomarker for Leishmania donovani

Philip J. R. Roche, Maurice C-K. Cheung, Mohamed Najih, Laura-Isobel McCall, Ibrahim Fakih, Vamsy P. Chodavarapu, Brian Ward, Momar Ndao, Andrew G. Kirk

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Proceedings Volume 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV; 82290C (2012) https://doi.org/10.1117/12.910146
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Visceral leishmaniasis (L.donovani) is a protozoan infection that attacks mononuclear phagocytes and causes the liver and spleen damage that can cause death. The investigation presented is a proof of concept development applying a plasmonic diagnostic platform with simple microfluidic sample delivery and optical readout. An immune-assay method is applied to the quantification of A2 protein, a highly immunogenic biomarker for the pathogen. Quantification of A2 was performed in the ng/ml range, analysis by ELISA suggested that a limit of 0.1ng/ml of A2 is approximate to 1 pathogen per ml and the sensing system shows the potential to deliver a similar level of quantification. Significant reduction in assay complexity as further enzyme linked enhancement is not required when applying a plasmonic methodology to an immunoassay. The basic instrumentation required for a portable device and potential dual optical readout where both plasmonic and photoluminescent response are assessed and investigated including consideration of the application of the device to testing where non-literate communication of results is considered and issues of performance are addressed.

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Philip J. R. Roche, Maurice C-K. Cheung, Mohamed Najih, Laura-Isobel McCall, Ibrahim Fakih, Vamsy P. Chodavarapu, Brian Ward, Momar Ndao, and Andrew G. Kirk "A study of a self diagnostic platform for the detection of A2 biomarker for Leishmania donovani", Proc. SPIE 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV, 82290C (14 February 2012); https://doi.org/10.1117/12.910146

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KEYWORDS

Plasmonics

Polymers

Luminescence

Nanorods

Calibration

Diagnostics

Gold

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