Mechanism of two-photon fluorescence increment via cross-linked bovine serum albumin

C.-Y. Lin; C.-H. Lien; C.-Y. Chang; S.-J Chen

doi:10.1117/12.930335

27 September 2012 Mechanism of two-photon fluorescence increment via cross-linked bovine serum albumin

C.-Y. Lin, C.-H. Lien, C.-Y. Chang, S.-J Chen

Proceedings Volume 8459, Physical Chemistry of Interfaces and Nanomaterials XI; 84590F (2012) https://doi.org/10.1117/12.930335
Event: SPIE NanoScience + Engineering, 2012, San Diego, California, United States

Abstract

The two-photon excited fluorescence (TPEF) increments of two dyes via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking technique were investigated. One is Rose Bengal (RB) with a high nonradiative decay rate, while the other is Eosin Y with a low non-radiative decay rate. Experimental results demonstrate that the quantum yield and lifetime of RB are both augmented via crosslinked BSA microstructures. Compared with theoretical analysis, this result indicates that the non-radiative decay rate of RB is decreased; hence, the quenched effect induced by BSA solution is suppressed. However, the fluorescence lifetime of Eosin Y is acutely abated despite the augmented quantum yield for the two-photon crosslinking processing from BSA solution. This result deduces that the radiative decay rate increased. Furthermore, the increased TPEF intensity and lifetime of RB correlated with the concentration of fabricated crosslinked BSA microstructures through pulse selection of the employed femtosecond laser is demonstrated and capable of developing a zone-plate-like BSA microstructure.

Citation Download Citation

C.-Y. Lin, C.-H. Lien, C.-Y. Chang, and S.-J Chen "Mechanism of two-photon fluorescence increment via cross-linked bovine serum albumin", Proc. SPIE 8459, Physical Chemistry of Interfaces and Nanomaterials XI, 84590F (27 September 2012); https://doi.org/10.1117/12.930335

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