3D high resolution photoacoustic imaging based on pure optical photoacoustic microscopy with microring resonator

Zhixing Xie; Chao Tian; Sung-Liang Chen; Tao Ling; Cheng Zhang; L. Jay Guo; Paul L. Carson; Xueding Wang

doi:10.1117/12.2041010

4 March 2014 3D high resolution photoacoustic imaging based on pure optical photoacoustic microscopy with microring resonator

Zhixing Xie, Chao Tian, Sung-Liang Chen, Tao Ling, Cheng Zhang, L. Jay Guo, Paul L. Carson, Xueding Wang

Author Affiliations +

Proceedings Volume 8943, Photons Plus Ultrasound: Imaging and Sensing 2014; 894314 (2014) https://doi.org/10.1117/12.2041010
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

For three-dimensional imaging of optical absorbance, the existing technology of photoacoustic microscopy (PAM) has quite poor axial resolution, the tens of microns to hundreds of microns. This is despite the fact that PAM has recently achieved lateral resolutions on the order of a micron or submicron, comparable to that of optical microscopy. In this paper, a pure optical photoacoustic microscopy (POPAM) with optical rastering of a focused excitation beam and optically sensing of the photoacoustic signal using a microring resonator was developed with the super broad bandwidth of the system more than 350MHz. With unprecedented broad bandwidth of POPAM, 3.8μm axial resolution was achieved without deconvolution processing. Sectioning imaging ability along axial direction presenting 3D morphologic features was shown based on imaging printed phantom. The impact of this approach will be similar to how confocal optical microscopy revolutionized the conventional optical microscopy by enabling the axial sectioning capability. Tissue imaging comparing POPAM and conventional PAM based on needle hydrophone demonstrated that though such broad bandwidth compromised the sensitivity of POPAM 4.35 times than that of conventional PAM, the noise equivalent detectable pressure (NEDP) was estimated as 74Pa, still able to get the tissue imaging.

Citation Download Citation

Zhixing Xie, Chao Tian, Sung-Liang Chen, Tao Ling, Cheng Zhang, L. Jay Guo, Paul L. Carson, and Xueding Wang "3D high resolution photoacoustic imaging based on pure optical photoacoustic microscopy with microring resonator", Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 894314 (4 March 2014); https://doi.org/10.1117/12.2041010

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available